To understand the inflammatory microenvironment and microbiome factors for the prognosis of chronic rhinosinusitis with polyps (CRSwNP), researchers explored the difference in characteristics of the microbiome of the nasal sinuses and inflammatory cytokines between recurrent and non-recurrent groups.
Scientists collected nasal secretions and polyp tissue from 77 CRSwNP patients. Then, they extracted microbial DNA from cotton swabs, performed high-throughput sequencing-based on 16S rRNA to detect bacterial community composition, and analyzed cytokines from polyp tissue.
Postoperative follow-up of patients with CRSwNP for 1 year was conducted to record the recurrence of nasal polyps and analyze the correlation between the recurrence of nasal polyps and the characteristics of inflammatory cytokines, inflammatory cell count, and nasal microbial diversity.
Postoperative recurrence of nasal polyps was not significantly correlated with age, sex, asthma, allergic rhinitis, or other allergic diseases in CRSwNP patients.
In terms of the total nasal symptom score, the recurrent group was significantly higher than the non-recurrent group.
In nasal polyp tissues, eosinophils and neutrophils in patients with CRSwNP in the recurrent group were significantly higher than those in the non-recurrent group, and neutrophils were also significantly higher in the recurrent group than the non-recurrent group.
The expression levels of IFN-, IL-17A, IL-17E, and IL-18 were significantly higher in the recurrent group than in the non-recurrent group, and the positive rates were not different.
In Southwest China, Enterobacteria and anaerobic bacteria may be correlated with the inflammatory pattern expression of nasal polyps. The neutrophil-mediated inflammatory response plays an important role in patients with CRSwNP in Southwest China and is correlated with nasal polyp recurrence.
Recurrence of nasal polyps after endoscopic sinus surgery may be potentially associated with a reduced abundance of protective microorganisms and an increased number of pathogenic microorganisms.
Published: 18 March 2021
source:
https://www.nature.com/articles/s41598-021-85292-5
https://doi.org/10.1038/s41598-021-85292-5
Commenti